NIGALA Aikeremu1,2, GAO Rui-juan1,3, TANG Xue-chun4, KONG Liang-jing-yuan1,2, GAO Yi-xuan1,2, YUAN Jia-yue1,2, MA ke-tao1,2, LI Li1,5, SI Jun-qiang1,2,6
1. The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University Medical College, Shihezi 832002;
2. Department of Physiology, Shihezi University Medical College, Shihezi 832002;
3. Department of Radiology, the First Affiliated Hospital of Shihezi University, Shihezi 832002;
4. Department of Burn and Plastic Surgery, the First Affiliated Hospital of Shihezi University, Shihezi 832002;
5. Department of Physiology, Jiaxing University Medical College, Jiaxing 314000;
6. Department of Physiology, Huazhong University of Science and Technology of Basic Medical Sciences, Wuhan 430030, China
Abstract: Objective: To investigate whether probenecid (PROB) could improve the proliferation and migration ability of rats' pulmonary artery smooth muscle cells induced by platelet-derived growth factor-BB (PDGF-BB). Methods: Primary pulmonary artery smooth muscle cells (PASMCs) of SD rats were cultured in vitro, and were randomly divided into control group (CON group), PDGF-BB group (10 ng/ml PDGF-BB treatment for 24 h) and PDGF-BB+PROB group (10 ng/ml PDGF-BB and 200 μmol/L PROB treatment for 24 h, PROB is a specific blocker of pannexin-1). CCK-8 method was used to select the suitable intervention concentrations of PROB and PDGF-BB, and to detect the proliferation of PASMCs in each group. The migration ability of PASMCs was detected by TranswellTM assay and cell scratch test. Immunofluorescence cytochemistry and Western blot were used to detect the protein expressions and distribution of osteopontin (OPN) and proliferating cell nuclear antigen (PCNA) in PASMCs. Results: Compared with CON group, the migration and proliferation ability of PASMCs in PDGF-BB group were enhanced (P＜0.05). After treated with PROB, the migration and proliferation ability of PASMCs in PDGF-BB+PROB group were decreased significantly (P＜0.05). Compared with CON group, the expression and protein levels of OPN and PCNA in PDGF-BB group were increased significantly (P＜0.05), while the expression and protein levels of OPN and PCNA in PDGF-BB+PROB were decreased significantly (P＜0.05). Conclusion: Probenecid inhibits the migration and proliferation of PDGF-BB-induced PASMCs by blocking Pannexin-1.
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Effects of probenecid on the migration and proliferation ability of platelet derived growth factor-BB induced pulmonary artery smooth muscle cells in rats.pdf